Skip to main content
. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Mol Immunol. 2012 Apr 7;51(2):234–244. doi: 10.1016/j.molimm.2012.03.025

Fig. 7. ELISA analysis of selected p35 and Ebi3 mutants.

Fig. 7

(A) Supernatants from HEK293T cells transfected with wild type or mutant p35 and either p40 or Ebi3 were assessed for cytokine levels by ELISA for IL-12 (anti-p35 C18.2 and anti-p40 biotin C17.8) and IL-35 (anti-p35 MMp35.8G7 and biotin anti-Ebi3 DV29). (B) Supernatants from HEK293T cells transfected with wild type or mutant Ebi3 and either p35 or p28 were assessed for soluble IL-27 or IL-35 levels by ELISA. (C) Supernatants from HEK293T cells transfected with wild type or patch mutant p35 and either p40 or Ebi3 were assessed for cytokine levels by ELISA for IL-12 or IL-35. Mean ± SEM from at least 3 independent experiments are shown as percent of wt IL-12 or IL-35. *p< 0.05, ** p< 0.01, *** p< 0.001