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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Mol Immunol. 2012 Apr 7;51(2):234–244. doi: 10.1016/j.molimm.2012.03.025

Fig. 8. Differential antibody epitope availability in IL-12 family cytokines.

Fig. 8

(A) Supernatants from HEK293T cells transfected with wild type IL-12 or IL-35 were subjected to immunoprecipitation by indicated anti-p35 monoclonal antibodies. Western blot analysis was performed using anti-p40 biotin (IL-12) or anti-Ebi3 biotin (IL-35). (B) Western blots from 3 independent experiments were quantified and plotted as percent of maximal signal. (C) Supernatants from cells transfected with wild type IL-27 or IL-35 were subjected to immunoprecipitation by indicated anti-Ebi3 monoclonal antibodies. The lower band in all Ebi3 western blots represents the light chain of the antibody used for immunoprecipitation. (D) Western blots from 3 independent experiments were quantified and plotted as percent of maximal signal.