Figure 3.
Measurement of extracellular ATP, a danger associated molecular pattern and a ligand for purinergic receptors. Extracellular ATP was measured from supernatants of CD-1 mouse primary hepatocytes at different time points co-cultured with 5 mM CCl4 (upper panel) and ATP release from hepatocytes (lower panel) that were isolated from lean and DIO mice. The hepatocytes were co-cultured with 5 mM CCl4 for 24 hours. ATP measurement was done on harvested cells at 24h. * Significantly different (P<0.05).