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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Free Radic Biol Med. 2012 Mar 3;52(9):1734–1743. doi: 10.1016/j.freeradbiomed.2012.02.032

Fig. 2.

Fig. 2

Overexpression or knockdown of PPARδ modulates Cox-2 and MCP-1 expression in BV-2 cells. A, BV-2 cells were transfected with 24 μg of the pcDNA3.1PPARδ vector or 24 μg of the empty pcDNA3.1 vector using Lipofectamine 2000 according to the manufacturer's protocol. Twenty-four h post-transfection, cells were serum starved for 24 h, treated for 3 h with 5 μM L-165041 or vehicle control, irradiated with a single dose of 10 Gy, and protein was harvested 7 h post-irradiation. Protein was subjected to western blot analysis for Cox-2; β-actin was used as a loading control. B, BV-2 cells were infected with scramble control or shRNA targeting PPARδ. Single knockdown clones were selected, irradiated with a single dose of 10 Gy, and protein was harvested 7 h post-irradiation. Protein was subjected to western blot analysis for Cox-2 or MCP-1. β-actin was used as a loading control.