Skip to main content
. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Free Radic Biol Med. 2012 Mar 3;52(9):1734–1743. doi: 10.1016/j.freeradbiomed.2012.02.032

Fig. 6.

Fig. 6

MEK1/2 or ERK1/2 inhibition prevents the radiation-induced increase in MCP-1, IL-1β, and TNF-α gene expression. BV-2 cells were pretreated with A, 2 μM U0126, B, 2 μM FR180204, or vehicle control for 3 h. Three h post-treatment, cells were irradiated with a single dose of 10 Gy and RNA was harvested 24 h post-irradiation. The expression levels of MCP-1, IL-1β, and TNF-α were analyzed using SYBER Green Real-time PCR and normalized with GAPDH expression levels. Changes in gene expression were calculated using the 2−ΔΔCt methods (see Materials and methods). Mean ± S.E.M; *, p≤ 0.05 vs. sham-irradiated, #, p≤ 0.05 vs. 10 Gy; n=3.