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SMase activity in human RBCs labeled with NBD-C6-SM. Cells were labeled with NBD-C6-SM, incubated in hypotonic (●) or isotonic (▴) buffer, and analyzed after elution in an HPLC column. (A) Variations of NBD-C6-ceramide. (B) Variations of NBD-C6-SM. Mean ± SD (n = 3).
