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Mass spectrometry analysis of different lipids in RBCs. Erythrocytes were incubated in hypotonic buffer at 37°C for 0 min (dark bars) and 6 min (gray bars). After lipid extraction, amounts of SM, PC, and PI were analyzed by mass spectrometry as described in Materials and Methods. ∗Significant difference from time 0 min (p < 0.05).
