TABLE 2.
| A. Fold Change of the Human Pathway-focused Osteogenesis Molecules Expressed by the ASCs Grown on the Ordered FA Surface Relative to the SSE Surfaces at Day 21 (Without OI) | ||||
|---|---|---|---|---|
| Symbol | Unigene | Refseq | Description Fold | Fold Change |
| COL10A1 | Hs.520339 | NM_000493 | Collagen, type X, alpha 1 | 4.14 |
| COMP | Hs.1584 | NM_000095 | Cartilage oligomeric matrix protein | 3.03 |
| FGF1 | Hs.483635 | NM_000800 | Fibroblast growth factor 1 (acidic) | 2.85 |
| TGFB3 | Hs.592317 | NM_003239 | Transforming growth factor, beta 3 | 3.41 |
| AMELY | Hs.1238 | NM_001143 | Amelogenin, Y-linked | −3.18 |
| BMP5 | Hs.296648 | NM_021073 | Bone morphogenetic protein 5 | −2.53 |
| CSF2 | Hs.1349 | NM_000758 | Colony stimulating factor 2 (granulocyte-macrophage) | −2.10 |
| CSF3 | Hs.2233 | NM_000759 | Colony stimulating factor 3 (granulocyte) | −8.63 |
| DMP1 | Hs.652366 | NM_004407 | Dentin matrix acidic phosphoprotein 1 | −2.13 |
| ICAM1 | Hs.643447 | NM_000201 | Intercellular adhesion molecule 1 | −3.48 |
| ITGAM | Hs.172631 | NM_000632 | Integrin, alpha M (complement component 3 receptor 3 subunit) | −2.83 |
| MMP9 | Hs.297413 | NM_004994 | Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) | −12.55 |
| STATH | Hs.654495 | NM_003154 | Statherin | −2.75 |
| TNF | Hs.241570 | NM_000594 | Tumor necrosis factor (TNF superfamily, member 2) | −4.32 |
| VEGFA | Hs.73793 | NM_003376 | Vascular endothelial growth factor A | −2.35 |
| B. Fold Change of the Human Pathway-focused Osteogenesis Molecules Expressed by the ASCs Grown on the Ordered FA Surface Relative to the SSE Surfaces at Day 21 (With OI) | ||||
|---|---|---|---|---|
| Symbol | Unigene | Refseq | Description | Fold Change |
| DMP1 | Hs.652366 | NM_004407 | Dentin matrix acidic phosphoprotein 1 | 2.39 |
| DSPP | Hs.678914 | NM_014208 | Dentin sialophosphoprotein | 2.27 |
| FGF1 | Hs.483635 | NM_000800 | Fibroblast growth factor 1 (acidic) | 129.25 |
| BMP2 | Hs.73853 | NM_001200 | Bone morphogenetic protein 2 | −2.09 |
| CALCR | Hs.489127 | NM_001742 | CALCITONIN RECEPTOR | −2.34 |
| FGFR2 | Hs.533683 | NM_000141 | Fibroblast growth factor receptor 2 | −2.06 |
| PDGFA | Hs.535898 | NM_002607 | Platelet-derived growth factor alpha polypeptide | −6.71 |
| VEGFA | Hs.73793 | NM_003376 | Vascular endothelial growth factor A | −2.14 |
After the real-time PCR amplification, relative gene expression values were analyzed using the Superarray web-based software package performing all ΔΔCt based fold-change calculations.