TABLE 3.
| A. Fold Change of the Human Pathway-focused Osteogenesis Molecules Expressed by the ASCs Grown on the Ordered FA Surface at Day 21 Relative to Day 7 (Without OI) | ||||
|---|---|---|---|---|
| Symbol | Unigene | Refseq | Description | Fold Change |
| BMP2 | Hs.73853 | NM_001200 | Bone morphogenetic protein 2 | 2.27 |
| COL10A1 | Hs.520339 | NM_000493 | Collagen, type X, alpha 1 | 2.99 |
| COL11A1 | Hs.523446 | NM_080629 | Collagen, type XI, alpha 1 | 3.04 |
| COL12A1 | Hs.101302 | NM_004370 | Collagen, type XII, alpha 1 | 2.06 |
| COL14A1 | Hs.409662 | NM_021110 | Collagen, type XIV, alpha 1 | 3.08 |
| COL15A1 | Hs.409034 | NM_001855 | Collagen, type XV, alpha 1 | 2.09 |
| COMP | Hs.1584 | NM_000095 | Cartilage oligomeric matrix protein | 6.69 |
| CTSK | Hs.632466 | NM_000396 | Cathepsin K | 2.30 |
| FGF1 | Hs.483635 | NM_000800 | Fibroblast growth factor 1 (acidic) | 2.85 |
| FGFR2 | Hs.533683 | NM_000141 | Fibroblast growth factor receptor 2 | 9.08 |
| IGF1 | Hs.160562 | NM_000618 | Insulin-like growth factor 1 (somatomedin C) | 3.16 |
| MMP8 | Hs.161839 | NM_002424 | Matrix metallopeptidase 8 (neutrophil collagenase) | 3.84 |
| TGFB3 | Hs.592317 | NM_003239 | Transforming growth factor, beta 3 | 2.47 |
| TGFBR1 | Hs.494622 | NM_004612 | Transforming growth factor, beta receptor 1 | 2.33 |
| VEGFA | Hs.73793 | NM_003376 | Vascular endothelial growth factor A | 2.68 |
| AHSG | Hs.324746 | NM_001622 | Alpha-2-HS-glycoprotein | −2.03 |
| AMBN | Hs.272396 | NM_016519 | Ameloblastin (enamel matrix protein) | −2.05 |
| AMELY | Hs.1238 | NM_001143 | Amelogenin, Y-linked | −3.60 |
| BMP3 | Hs.387411 | NM_001201 | Bone morphogenetic protein 3 | −2.44 |
| BMP5 | Hs.296648 | NM_021073 | Bone morphogenetic protein 5 | −2.58 |
| CSF2 | Hs.1349 | NM_000758 | Colony stimulating factor 2 (granulocyte-macrophage) | −2.38 |
| CSF3 | Hs.2233 | NM_000759 | Colony stimulating factor 3 (granulocyte) | −2.60 |
| ENAM | Hs.667018 | NM_031889 | Enamelin | −3.60 |
| IGF2 | Hs.523414 | NM_000612 | Insulin-like growth factor 2 (somatomedin A) | −2.29 |
| ITGAM | Hs.172631 | NM_000632 | Integrin, alpha M (complement component 3 receptor 3 subunit) | −2.69 |
| MMP9 | Hs.297413 | NM_004994 | Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) | −3.09 |
| TNF | Hs.241570 | NM_000594 | Tumor necrosis factor (TNF superfamily, member 2) | −2.39 |
| B. Fold Change of the Human Pathway-focused Osteogenesis Molecules Expressed by the ASCs Grown on the Ordered FA Surface at Day 21 Relative to Day 7 (With OI) | ||||
|---|---|---|---|---|
| Symbol | Unigene | Refseq | Description | Fold Change |
| AHSG | Hs.324746 | NM_001622 | Alpha-2-HS-glycoprotein | 15.05 |
| AMELY | Hs.1238 | NM_001143 | Amelogenin, Y-linked | 2.09 |
| BGN | Hs.821 | NM_001711 | Biglycan | 2.66 |
| BMP5 | Hs.296648 | NM_021073 | Bone morphogenetic protein 5 | 2.83 |
| CALCR | Hs.489127 | NM_001742 | CALCITONIN RECEPTOR | 11.89 |
| CDH11 | Hs.116471 | NM_001797 | Cadherin 11, type 2, OB-cadherin (osteoblast) | 2.68 |
| COL10A1 | Hs.520339 | NM_000493 | Collagen, type X, alpha 1 | 6.69 |
| COL14A1 | Hs.409662 | NM_021110 | Collagen, type XIV, alpha 1 | 6.69 |
| COL15A1 | Hs.409034 | NM_001855 | Collagen, type XV, alpha 1 | 6.03 |
| COMP | Hs.1584 | NM_000095 | Cartilage oligomeric matrix protein | 2.87 |
| CSF2 | Hs.1349 | NM_000758 | Colony stimulating factor 2 (granulocyte-macrophage) | 2.03 |
| CTSK | Hs.632466 | NM_000396 | Cathepsin K | 4.20 |
| DMP1 | Hs.652366 | NM_004407 | Dentin matrix acidic phosphoprotein 1 | 3.12 |
| DSPP | Hs.678914 | NM_014208 | Dentin sialophosphoprotein | 3.21 |
| EGF | Hs.419815 | NM_001963 | Epidermal growth factor (beta-urogastrone) | 2.74 |
| FGF1 | Hs.483635 | NM_000800 | Fibroblast growth factor 1 (acidic) | 2.45 |
| FGF3 | Hs.37092 | NM_005247 | Fibroblast growth factor 3 (murine mammary tumor virus integration site (v-int-2) oncogene homolog) | 2.18 |
| ICAM1 | Hs.643447 | NM_000201 | Intercellular adhesion molecule 1 | 3.51 |
| IGF1 | Hs.160562 | NM_000618 | Insulin-like growth factor 1 (somatomedin C) | 62.77 |
| IGF1R | Hs.643120 | NM_000875 | Insulin-like growth factor 1 receptor | 2.16 |
| IGF2 | Hs.523414 | NM_000612 | Insulin-like growth factor 2 (somatomedin A) | 12.40 |
| MMP2 | Hs.513617 | NM_004530 | Matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) | 2.87 |
| MMP8 | Hs.161839 | NM_002424 | Matrix metallopeptidase 8 (neutrophil collagenase) | 2.12 |
| TGFBR2 | Hs.604277 | NM_003242 | Transforming growth factor, beta receptor II (70/80kDa) | 2.27 |
| VCAM1 | Hs.109225 | NM_001078 | Vascular cell adhesion molecule 1 | 17.78 |
| ENAM | Hs.667018 | NM_031889 | Enamelin | −2.51 |
| GDF10 | Hs.2171 | NM_004962 | Growth differentiation factor 10 | −2.46 |
| MSX1 | Hs.424414 | NM_002448 | Msh homeobox 1 | −2.05 |
| PDGFA | Hs.535898 | NM_002607 | Platelet-derived growth factor alpha polypeptide | −6.49 |
After the real-time PCR amplification, relative gene expression values were analyzed using the Superarray web-based software package performing all ΔΔCt based fold-change calculations.