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SEC profiles of FtsZ at 25°C monitored by the absorbance at 220 nm. The samples at 3 μM (solid line) and 40 μM (dashed line) were equilibrated in the mobile phase and then injected into the column. Elution peaks corresponding to monomer, dimer, and tetramer species are marked as M, D, and T, respectively. A small fraction (<1%) of high-molecular-mass aggregates eluted in the void volume, indicated by an arrow in the main plot. The inset shows reinjection of the monomer fraction.
