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. 2012 May 2;102(9):2176–2185. doi: 10.1016/j.bpj.2012.03.064

Figure 2.

Figure 2

Fluorescence anisotropy of FtsZ conjugates as a function of protein concentration in 50 mM potassium phosphate buffer, pH 6.5, at 25°C. (A) Dilution of FtsZ-FITC conjugates. (B) Dilution of FtsZ-DNS conjugates. The experimental data (symbols) were fit to the equilibrium of dissociation of a tetramer into monomers through a dimer intermediate 2M↔D, 2D↔T (solid lines); limiting anisotropies and dissociation constants are summarized in Table 1. The error bars represent the standard deviation (SD) of the 20 iterations used to calculate the anisotropy.