Table 2.
FITC conjugates∗ |
|||||
---|---|---|---|---|---|
FtsZ μM | τave. | θ1 | r1 | θ2 | r2 |
31 | 3.68 | 0.57† (0.05) | 0.16 (<0.01) | 38.9 (5.7) | 0.15 (<0.01) |
6 | 3.68 | 0.48 (0.04) | 0.18 (<0.01) | 34.6 (5.0) | 0.13 (<0.01) |
1 | 3.58 | 0.29 (0.03) | 0.19 (<0.01) | 19.7 (1.9) | 0.12 (0.01) |
DNS conjugates |
|||||
FtsZ μM | τave. | θ1 | r1 | θ2 | r2 |
45 | 15.2 | 0.54 (0.25) | 0.07 (0.03) | 147 (5) | 0.27 (<0.01) |
4 | 17.3 | 1.04 (0.23) | 0.05 (<0.01) | 67 (2) | 0.29 (<0.01) |
0.5 | 17.6 | 0.99 (0.24) | 0.03 (<0.01) | 46 (1) | 0.31 (<0.01) |
Global reduced χ2-values of the fits were 0.67 and 0.35 for FITC and DNS conjugates, respectively.
Constant standard errors of 0.2° for phase angle and 0.004 for modulation were used for minimization. Values in parentheses are the standard errors of the fit parameters.