Table 3.

Thermodynamic analysis of the unfolding of FtsZ using the three-state dimer model

FtsZ∗μM	$\Delta G_{H_{2}O,N_2\leftrightarrow I_2}^0$ kcal mol−1	$m_{N_2\leftrightarrow I_2}$ kcal mol-1M−1	$C_{50\%,N_2\leftrightarrow I_2}$ M†	$\Delta G_{H_{2}O,I_2\leftrightarrow 2U}^0$ kcal mol−1	$m_{I_2\leftrightarrow 2U}$ kcal mol-1M−1	$C_{50\%,I_2\leftrightarrow 2U}$ M	$\Delta G_{T{H}_{2}O}^0$ kcal mol−1
1	2.3 (1.9)‡	7.2 (4.2)	0.32	11.1 (0.8)	2.5 (0.4)	1.2	13.4 (2.6)
5.6	2.5 (1.8)	10.5 (6.6)	0.24	10.1 (0.2)	1.8 (0.1)	1.7	12.6 (2.1)
46.3	1.7 (0.9)	7.1 (3.1)	0.25	12.8 (0.3)	3.3 (0.1)	2.1	14.5 (1.3)
GA§	1.8 (0.6)	6.6 (2.0)	—	12.2 (0.4)	3.2 (0.1)	—	14.0 (1.1)

^∗Reduced χ²-values of the fits were 1.3, 1.5, 1.8, and 2.2, respectively.

^†C_50% values were calculated as described in the Supporting Material and Materials and Methods.

^‡Values in parentheses are the fit standard errors.

^§GA indicates the results obtained with the full data sets by global analysis.