Fig. 3.

ADH1 activity in T. vaginalis homogenates. The first bar (iron+) represents the acetaldehyde reduction rate in homogenates from cells grown in the presence of supplemented iron. The second and third bars represent the acetaldehyde production rate in cells grown without supplemented iron, either without (iron−) or with (iron−/ZnCl₂+) 0.5 mM ZnCl₂ added to the homogenates prior to the assay. Values were determined in at least two independent experiments. For comparison, the rate of expression of ADH1 is given as percentage of total protein visualized by 2DE (Fig. 2) below the bars.