Table 1.

Effects of culture factors on cell growth and reduction activity of Acetobacter sp. CCTCC M209061

Factor	Biomass (g/L)	Specific activity (μmol/min/g)	e.e. (%)
Extra vitamins
Control	0.94 ± 0.04	34.15 ± 1.05	≥ 99
Vitamin B1	0.93 ± 0.03	34.36 ± 0.94	≥ 99
Nicotinic acid	0.93 ± 0.01	35.04 ± 1.07	≥ 99
Pyridoxine	0.95 ± 0.02	34.18 ± 0.89	≥ 99
Ascorbic acid	0.94 ± 0.03	33.88 ± 0.93	≥ 99
Biotin	0.93 ± 0.04	34.59 ± 0.86	≥ 99
pH
4	0.38 ± 0.01	11.61 ± 0.39	≥ 99
5	0.95 ± 0.02	34.67 ± 1.10	≥ 99
6	0.94 ± 0.04	34.15 ± 1.05	≥ 99
7	0.82 ± 0.03	29.37 ± 0.84	≥ 99
8	0.48 ± 0.01	23.03 ± 0.58	≥ 99
Temperature (°C)
25	0.79 ± 0.03	33.01 ± 1.10	≥ 99
30	0.95 ± 0.02	34.67 ± 1.10	≥ 99
35	0.86 ± 0.02	31.38 ± 1.05	≥ 99
40	0.62 ± 0.02	23.21 ± 0.61	≥ 99
45	0.08 ± 0.01	0	-
Shaking rate (rpm)
40	0.76 ± 0.02	39.55 ± 1.12	≥ 99
80	0.96 ± 0.02	36.76 ± 1.03	≥ 99
100	0.95 ± 0.02	34.67 ± 1.10	≥ 99
120	0.97 ± 0.02	30.14 ± 0.99	≥ 99
160	0.96 ± 0.03	24.24 ± 0.65	≥ 99
200	0.97 ± 0.04	17.74 ± 0.47	≥ 99