Figure 2.

Overexpression of DTIS11 proteins in the Drosophila eye. (A-D) Bright field images of fly retinas expressing TIS11 variants by GMR-Gal4. (A, control; B, Drosophila TIS11 [DTIS11]; C, murine TIS11b [mTIS11b]; D, RNA-binding domain mutant DTIS11^F158N). (E) Use of the pigment cell driver Lz-Gal4 to express DTIS11 in retina. Pupal eyes (48 h APF) were dissected and labeled by immunohistochemistry (left, control; right, DTIS11 overexpression). Retinal pigment cells were revealed by anti-armadillo staining (green), and rhabdomere structure of photoreceptors was counterstained with phalloidin (red). Scale bar, 10 μm. (F) Immunoblot analysis of total protein from fly heads for expression of DTIS11 and DTIS11^F158N. w¹¹¹⁸ was used as a non-transgenic control. Numbers below bands indicate amount of protein loaded.