Figure 3.

Identification of eya mRNA as a target of TIS11 proteins in Drosophila. (A) Analysis of eya expression. Semi-quantitative RT-PCR determines the mRNA expression of eya (top) and rp49 (middle), and the level of eya mRNA bound to FLAG-tagged TIS11b (bottom) in control and mouse TIS11b overexpression fly. Numbers indicate amount of RNA-IP reaction analyzed by quantitative PCR. Right panel is immunoblotting analysis with anti-Eya. (B) Eye phenotype resulting from DTIS11 overexpression was similar to the eya mutant. (C) Coexpression of eya with mTIS11b partially rescued the small eye phenotype caused by overexpression of mTIS11b alone. (D) Statistical analysis shows that eya significantly rescued the reduced eye size resulting from mTIS11b overexpression. n = 3; *P = 0.0048 by two-tailed Student's t-test.