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. 2012 Apr 20;8(5):606–619. doi: 10.7150/ijbs.3782

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Overexpression of DTIS11 in S2 cells. (A) Overexpression of DTIS11 decreased eya mRNA expression in S2 cells. S2 cells were transfected with GFP-tagged DTIS11 (WT) or GFP-tagged DTIS11^F158N (F158N) expression plasmids. eya expression was determined by quantitative PCR and normalized to rp49 as a negative control (*P < 0.05, **P < 0.01). Levels of GFP-tagged proteins were detected by immunoblotting with anti-GFP. (B) Cell viability analysis. S2 cells were seeded into 96-well plates and transiently transfected with GFP-tagged DTIS11 or DTIS11^F158N expression plasmids. After 2 days, cell viability was determined by MTS assay and normalized to vector control (**P < 0.01, ***P < 0.001). Each treatment was performed in triplicate and the experiment was repeated five times.

Overexpression of DTIS11 in S2 cells. (A) Overexpression of DTIS11 decreased eya mRNA expression in S2 cells. S2 cells were transfected with GFP-tagged DTIS11 (WT) or GFP-tagged DTIS11^F158N (F158N) expression plasmids. eya expression was determined by quantitative PCR and normalized to rp49 as a negative control (*P < 0.05, **P < 0.01). Levels of GFP-tagged proteins were detected by immunoblotting with anti-GFP. (B) Cell viability analysis. S2 cells were seeded into 96-well plates and transiently transfected with GFP-tagged DTIS11 or DTIS11^F158N expression plasmids. After 2 days, cell viability was determined by MTS assay and normalized to vector control (**P < 0.01, ***P < 0.001). Each treatment was performed in triplicate and the experiment was repeated five times.