Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Apr 27;8(5):630–639. doi: 10.7150/ijbs.3684

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

PMC Copyright notice

Fig 2 — Immunoblot assays show the developmental regulation and glycosylation of β4 subunit. (A) The ~38 kDa band was not expressed until postnatal day 7 in wild-type mice. (B) The treatment of the membrane fractions from the P7, P28 and adult brain tissues with glycosidase PNase F (Enz) converted the immunoreactive proteins into a faster migrating species. P0 and P6 show the immunoreactive proteins without changes between the treated and untreated samples. (C) The similar treatment of the membrane fractions from neonatal A53T mice brain tissues converted the ~38 kDa protein into a faster migrating species, but there is no change in the 35 kDa band with or without PNGase F treatment. The + and - signs indicate the treated and untreated samples, respectively. WT: wild-type mice; A53T: PD transgenic mice; Ad: adult wild-type mice.