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. 2012 Jan 20;166(5):941–949. doi: 10.1530/EJE-11-0849

Figure 2.

Figure 2

mRNA levels of total CYP19 and CYP19 promoter-specific transcript variants. Transcript levels of total aromatase (CYP19) and promoter-specific CYP19 transcript variants were determined in both tumour (T) and normal (N) testes with qPCR. Triplicate runs were performed, and expression of the ribosomal protein P2 (RPLP2) was used as reference in all the reactions. Individual in-run standard curves were used in the five different assays and the relative concentrations as displayed on the y-axes are therefore not comparable between the assays A, B, C, D and E. We observed an increased transcript level of (A) total CYP19 (125-fold), (B) promoter II-specific CYP19 (59-fold), (C) promoter 1.3-specific CYP19 (11-fold) and (E) promoter 1.7-specific CYP19 (not detected in the normal sample). (D) We observed a down-regulation of promoter 1.4-specific CYP19 transcript level (tumour to normal ratio: 0.4).