Table 2.
Primers and probes.
| Primers/probes | Sequences |
|---|---|
| mRNA expression | |
| Total CYP19 F | 5′-ATCCTCAATACCAGGTCCTGGC |
| Total CYP19 R | 5′-AGAGATCCAGACTCGCATGAATTCT |
| Total CYP19 probe | 5′-6FAM-ACCCGGTTGTAGTAGTTGCAGGCACT-BBQ |
| RPLP2 F | 5′-GACCGGCTCAACAAGGTTAT |
| RPLP2 R | 5′-CCCCACCAGCAGGTACAC |
| RPLP2 probe | 5′-Cy5-AGCTGAATGGAAAAAACATTGAAGACGTC-BBQ |
| CYP19_exon1.3 F | 5′-GTCTAAAGGAACCTGAGACTCTACC |
| CYP19_exon1.4 F | 5′-CACTGGTCAGCCCATCAA |
| CYP19_exon1.7 F | 5′-AGAAAGGGGTGAAATCAGCAA |
| CYP19_exon1 common R | 5′-ACGATGCTGGTGATGTTATAATGT |
| CYP19_exon1 common probe | 5′-6FAM-TCGGGTTCAGCATTTCCAAAACCAT-BBQ |
| CYP19_exonII F | 5′-CCCTTTGATTTCCACAGGAC |
| CYP19_exon3 R | 5′-CCCATGCAGTAGCCAGGAC |
| CYP19_exonII probe | 5′-6FAM-CATGCCAGTCCTGCTCCTCA-BBQ |
| COX2 F | 5′-ATCACAGGCTTCCATTGACC |
| COX2 R | 5′-CAGGATACAGCTCCACAGCA |
| COX2 probe | 5′-6FAM-CCGCAAACGCTTTATGCTGA-BBQ |
| SF-1 F | 5′-TCATCCTCTTCAGCCTGGAT |
| SF-1 R | 5′-AGGTACTCCTTGGCCTGCAT |
| SF-1 probe | 5′-6FAM-ACGCTCAGGAGAAGGCCAAC-BBQ |
| LRH-1 F | 5′-GCACAGGAGTTAGTGGCAAA |
| LRH-1 R | 5′-CTGCTGCGGGTAGTTACACA |
| LRH-1 probe | 5′-6FAM-AACAAGTCAATGCCGCCCT-BBQ |
| Gene copy number analysis | |
| CYP19 gDNA F1 | 5′-TTATGGGAGCCTGGTAGTGG |
| CYP19 gDNA R1 | 5′-AGGGTGGGGTTTTCTATTGG |
| CYP19 gDNA probe 1 | 5′-6FAM-TTGGAACATCATACCCCGGG-BBQ |
| CYP19 gDNA F2 | 5′-GGTGGCTTTTGCTGATCAAT |
| CYP19 gDNA R2 | 5′-AGAAAATGGGCACGAAACTG |
| CYP19 gDNA probe 2 | 5′-6FAM-AGCTCCTGCCAGCTCCCTTT-BBQ |
| CYP19 PII gDNA F1 | 5′-TTGGTCAAAAAAGGGGAGTTG |
| CYP19 PII gDNA R1 | 5′-ATCATCTTGCCCTTGAGTGG |
| CYP19 PII gDNA F2 | 5′-TCCACCTCTGGAATGAGCTT |
| CYP19 PII gDNA R2 | 5′-TTGCAGCATTTCTGACCTTG |
| CYP19 PII gDNA probes 1 and 2 | 5′-6FAM-CTTTCAATTGGGAATGCACG-BBQ |
| CYP19 PII gDNA F3 | 5′-GGGCTTCCTTGTTTTGACTT |
| CYP19 PII gDNA R3 | 5′-GAGGGGGCAATTTAGAGTCC |
| CYP19 PII gDNA probe 3 | 5′-6FAM-CACCCTCTGAAGCAACAGGA-BBQ |
| B2M gDNA F | 5′-CATCCAGCAGAGAATGGAAAG |
| B2M gDNA R | 5′-GAAAGACCAGTCCTTGCTGAA |
| B2M gDNA probe | 5′-Cy5-TGGGTTTCATCCATCCGACA-BBQ |
| PCR/sequencing of CYP19 PII and SF-1 promoter area | |
| CYP19 PII PCR F1 | CTCAACGATGCCCAAGAAAT |
| CYP19 PII PCR R1 | CATGGACCAAAATCCCAAGT |
| CYP19 PII Seq F2 | TTGGTCAAAAAGGGGAGTTG |
| CYP19 PII Seq R2 | CTTACCTGGTATTGAGGATGTGC |
| CYP19 PII Seq F3 | GGCAAGAAATTTGGCTTTCA |
| CYP19 PII Seq R3 | GAGGGGGCAATTTAGAGTCC |
| SF-1 F2 | TCTGCCTCCCAGGTTCAAGC |
| SF-1 R8 | GGTGACTGGATCTTTTGTGTGTTCCTC |
| SF-1 F4 | GAGGAACACACAAAAGATCCAGTCACC |
| SF-1 R7 | CTTGGCCAGCTGGCTGTTG |
| SF-1 F6 | TGTCCTGACTCTACTCCAATGTCCG |
| SF-1 R2 | AACCCCTGAGAAACAAGAGCATCTG |
| SF-1 R9 (seq) | TGTGTGTGTTCCTCTGTTTGTCTCAC |
| SF-1 R10 (seq) | GGGCTTGATTTATGGGCTGCTC |
| SF-1 F6 (seq) | TGTCCTGACTCTACTCCAATGTCCG |
| SF-1 F8 (seq) | CACCAACAAAGAAGGCGAGAGG |
| SF-1 F9 (seq) | TCTGTCCCCCACCTGAGTTTC |
| SF-1 F10 (seq) | CCACTGCCACCCTCATCC |