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. Author manuscript; available in PMC: 2012 Oct 12.
Published in final edited form as: Nature. 2012 Apr 11;484(7393):251–255. doi: 10.1038/nature10985

Figure 1. Development of transcription factor competition-ChIP in yeast.

Figure 1

(a) Schematic of Rap1 competition-ChIP yeast strain. (b) Growth comparison of competition yeast strain to wild-type in inducing (2% Galactose) and non-inducing (2% Dextrose) conditions. (c) Western blot using an antibody against Rap1 (y-300). Strains containing only a Rap1-Myc or only Rap1-Flag copy are shown to the right to indicate the size of isoform-specific bands. Actin loading control below. (d) To estimate the dynamics of induction, the ratio of induced Rap1-Myc and constitutive Rap1-Flag protein is plotted. Data is from two technical replicates of two independent time course replicates. Error bars represent standard error.