Figure 3.
Confocal images of TUNEL stained HEK293T cells transfected with either WT or mutant DFNA5-EGFP. Red stained nuclei are TUNEL positive. Cells transfected with WT DFNA5 display a healthy appearance with little to no TUNEL positive nuclei. In contrast, cells transfected with mutant DFNA5 display an unhealthy appearance with clear TUNEL positive nuclei, indicative of apoptotic cell death.