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. Author manuscript; available in PMC: 2013 Apr 20.
Published in final edited form as: Biochem Biophys Res Commun. 2012 Mar 17;420(4):727–732. doi: 10.1016/j.bbrc.2012.03.050

Figure 1. HEK293 cells have Cco/NO activity.

Figure 1

(A) HEK293 cell lysates were prepared either with (dashed line) or without (solid line) phosphatase inhibitor. NO production was measured under anoxic conditions with the Innovative Instruments inNOII system as described in Materials and Methods using 3 mg/mL cell lysate. 1 mM NaNO2 was added as indicated. The NO scavenger, PTIO, was added at the time indicated to confirm the specificity of the observed signal. (B) The effects of sodium azide (5 mM; long dashed line) or L-NAME (1 mM; short dashed line) on Cco/NO activity in phosphatase inhibitor- treated HEK293 cell lysates were measured as described in Panel (A).