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. 2012 Apr 3;106(9):1512–1519. doi: 10.1038/bjc.2012.126

Figure 1.

Figure 1

Enrichment of CD133+ CSCs and CD133− NSCCs through FACS. (A–C) typical image of FACS sorting and efficiency. (A) Sorting of CD133+ and CD133− cell subpopulation. (B) Typical enrichment efficiency of CD133+ subpopulation from A. (C) Typical enrichment efficiency of CD133− subpopulation from A. (D–F) Sphere formation of CD133+ and CD133− cells. (D) Typical image of sphere formations of CD133+ cells. (E) Typical image of sphere formations of CD133− cells, bar=100 μm. (F) Statistics in (D and E) at day 4 post inoculation (mean±s.d., n=3; **P=0.00109).