Figure 4.
Inhibition of the PI3K/Akt pathway reduced peritoneal metastasis in nude mice. (A) The frequency of metastasis, number and total volume of nodules/mouse were identified. Upper lane: after cells were injected 2 weeks and 4 weeks with the treatment of 25 mg kg−1 LY294002. Lower lane: after cells were injected 4 weeks with the treatment of 25 mg kg−1 and 50 mg kg−1 of LY294002. *P<0.05 vs control. (DMSO treatment). Values shown are mean±s.d. (n=5). (B) Effect of p85 expression on incidence of grossly visible peritoneal metastatic nodules. *P<0.05 vs Wp85-transfected subclones. Values shown are mean±s.d. (n=10).