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. Author manuscript; available in PMC: 2012 May 2.
Published in final edited form as: Bioorg Med Chem Lett. 2011 May 18;21(17):4945–4950. doi: 10.1016/j.bmcl.2011.05.045

Figure 2.

Figure 2

(A) Selective enrichment and identification of cell surface azidoglycoproteins by live-cell Staudinger ligation with Phosphine-FLAG. Labeled proteins are captured by immunoprecipitation of the FLAG tag, followed by SDS–PAGE. Gel bands are then excised and analyzed by LC–MS/MS. (B) PC-3 cells and (C) PC tissue slice cultures were labeled with GalNAz or GalNAc. The lysates were treated with Phos-FLAG and analyzed by Western blotting with anti-FLAG antibody. Very little background labeling was apparent in the control cells (treated with GalNAc).