Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Apr 2;3:62. doi: 10.3389/fimmu.2012.00062

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2012 Zinöcker and Vaage.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

PMC Copyright notice

MSC express iNOS and produce NO in response to lymphocyte stimulation. (A) 1 × 10⁵ ConA-stimulated PVG LNC were cultured in the absence (CTR) or presence of syngeneic PVG.7B MSC [log (MSC:LNC)] and expression of iNOS was analyzed after 24 h. iNOS was detected in co-cultures of ConA–LNC with MSC and correlated with the numbers of MSC present. (B) In the same set-up as shown in (A) the amount of nitrate (shown as the mean and the SD of triplicates) as a proxy for NO concentration was determined in the absence (□) or presence () of L-NMMA. Addition of L-NMMA abolished NO levels produced in MSC:LNC co-cultures. NO was not detected in the absence of MSC (CTR). Data are representative of three independent experiments.

Inline graphic — MSC express iNOS and produce NO in response to lymphocyte stimulation. (A) 1 × 10⁵ ConA-stimulated PVG LNC were cultured in the absence (CTR) or presence of syngeneic PVG.7B MSC [log (MSC:LNC)] and expression of iNOS was analyzed after 24 h. iNOS was detected in co-cultures of ConA–LNC with MSC and correlated with the numbers of MSC present. (B) In the same set-up as shown in (A) the amount of nitrate (shown as the mean and the SD of triplicates) as a proxy for NO concentration was determined in the absence (□) or presence () of L-NMMA. Addition of L-NMMA abolished NO levels produced in MSC:LNC co-cultures. NO was not detected in the absence of MSC (CTR). Data are representative of three independent experiments.