Skip to main content
. 2011 Jul 4;2:26. doi: 10.3389/fimmu.2011.00026

Figure 3.

Figure 3

NK1.1+CD11c+ NK cells are main IFN-γ-producing cells in both the spleen of mice infected with LM for 20 h and the in vitro 20-h coculture of NWNA spleen cells with LM-infected BMM cells. (A) Splenocytes from uninfected mice were stained for NK1.1, CD11c, and either CD3, CD244, or B220, and three-color flow-cytometric analysis was performed on FACScan. (B) Splenocytes from LM-infected mice were stained for NK1.1, CD11c, and either intracellular IFN-γ or B220. (C–E) NWNA spleen cells before culture (C) and after coculture with uninfected (D) or LM-infected (E) BMM cells were stained for NK1.1, CD11c, and either intracellular IFN-γ or one of the following surface markers: CD3, CD244, and B220. Representative data from one of three independent experiments are shown. The numbers shown in or outside quadrants represent mean percentages of cells in total splenocytes and the numbers within histograms represent mean percentages of cells expressing surface antigen indicated or intracellular IFN-γ in gated cell populations from three independent experiments.