Figure 5.

Uptake of LM by ER-TR9⁺ MZMs and formation of infectious foci without being associated with MZMs. Cryosections of the spleen taken from mice inoculated with LM and fluorescent microsphers (blue) were stained for LM (red) and MZMs (green). (A) Four-hour postinfection; fluorescent microsphers and MZMs delineate the MZ and a few bacteria (arrows) are associated with MZMs. (B) Twenty-hour postinfection; bacteria form infectious foci. MZMs are found around infectious foci, but most of bacteria are no longer associated with MZMs. Insets are enlarged squares shown in (A,B). Some bacteria are also found in the interior of the white pulp. Bar in (A) = 100 μm for both (A,B). Bar in [(A) inset] = 20 μm for both insets. Representative sections are shown.