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. 2011 Jul 4;2:26. doi: 10.3389/fimmu.2011.00026

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Copyright © 2011 Kubota and Kadoya.

This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and other Frontiers conditions are complied with.

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Infectious foci are formed in close vicinity of marginal zone area. Cryosections of the spleens taken from mice 16 h (A) and 20 h (B) after the inoculation of LM and fluorescent microspheres (blue) were stained for perlecan (green) and LM (red). Perlecan is expressed in the basement membranes surrounding the marginal sinuses and blood vessels. Arrows indicate infectious foci. Some bacteria are also found in the interior of the white pulp in (B). CA, central arteriole. Bar = 100 μm. Representative sections are shown.