Figure 7.

Interferon-γ-producing cells are localized to infectious foci. Serial formaldehyde-fixed sections of the spleen taken from mice 20 h after the inoculation of LM and fluorescent microsphers (blue) were separately stained for LM [(A,C) red] and IFN-γ [(B,D) red], respectively. Consecutive areas were photographed. The squares shown in (A,B) were enlarged in (C,D). Bacteria and IFN-γ-producing cells are visualized as red spots and cytoplasmic staining, respectively. Bar = 100 μm for (A,B), 50 μm for (C,D). Representative consecutive sections are shown.