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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: J Cell Physiol. 2012 Mar;227(3):1062–1070. doi: 10.1002/jcp.22819

Figure 5. BaP stimulates chondrocyte maturation in Stage E12 limb bud chondrocytic cells.

Figure 5

More mature limb bud cells harvested at stage E12 were cultured in micromass. Cultures were treated with 1μM BaP and mRNA was harvested at indicated time points. qPCR was performed to quantify the expression of col2 (A), colX (B) and ALP (C). qPCR results were corrected for β-actin expression and normalized to the day 1 level for each gene (N=3, †p=0.071, *p<0.05, **p<0.01, Student’s t-test).