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. 2011 Mar 21;2:6. doi: 10.3389/fimmu.2011.00006

Figure 4.

Figure 4

CD25 expression on a subset of B1a cells is not accompanied by expression of CD122. Five lymphocyte populations were sort-purified: splenic follicular B2 cells (“B2”), peritoneal B1a cells (“B1”), CD25− peritoneal B1a cells (“CD25− B1”), CD25+ peritoneal B1a cells (“CD25+ B1”), and splenic T cells (“T”). B cell populations were unstimulated whereas T cells were stimulated by ConA at 5 μg/ml for 2 days. RNA was prepared, reverse transcribed, and evaluated for expression of CD122 by real-time PCR, normalized to β2-microglobulin. Expression by each population relative to peritoneal B1a cells is shown as the mean of six experiments with lines indicating the SE of the means.