Figure 5.

CD25⁺ B1a cells express LIF receptor (LIFR). Five lymphocyte populations were sort-purified: splenic follicular B2 cells (“B2”), peritoneal B1a cells (“B1”), CD25⁻ peritoneal B1a cells (“CD25⁻ B1”), CD25⁺ peritoneal B1a cells (“CD25⁺ B1”), and splenic T cells (“T”). (A) RNA was prepared from each of these populations, reverse transcribed, and evaluated for expression of LIFR by real-time PCR, normalized to β2-microglobulin. Expression by each population relative to peritoneal B1a cells is shown as the mean of six experiments with lines indicating the SE of the means, with the exception of splenic T cells for which n = 3. (B) Protein extracts were prepared from each of these populations and immunoblotted for expression of “LIFR”. Blots were stripped and reprobed for expression of total beta actin (actin) as a loading control. Representative results from one of three comparable experiments are shown.