Figure 3.

Effect of costimulatory molecule deficiency on the presence of CD4 and CD8 T cell in Lat^Y136F mutant mice. (A) The frequency of CD4 and CD8 T cells present in the lymph node of 8-week-old WT (upper row) or of Lat^Y136F mice (lower row) lacking the costimulatory molecule specified above the dot plots was assessed by flow cytometry (B) T cells from the spleen of the specified 8-week-old mice were analyzed for the expression of CD4 and CD8. The percentage of cells is shown in each quadrant [upper row (C)]. Total number of CD4 and CD8 T cells present in pooled inguinal, brachial, and axillary lymph nodes (left column) and in the spleen (right column) of WT (black bar) and Lat^Y136F mice (open bar) lacking the indicated costimulatory molecules on the surface of B cells. (D) Absolute numbers of CD4 and CD8 T cells recovered per spleen of 8-week-old mice lacking the indicated costimulatory molecule on the surface of T cells. Histogram corresponds to the mean ± SD of at least three mice analyzed in independent experiments. t-Test or Mann Whitney test was performed to determine whether differences were significant. *p-Value < 0.05; **p-value < 0.01.