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. 2012 Mar 5;3:38. doi: 10.3389/fimmu.2012.00038

Figure 3.

Figure 3

Surface levels of C1 complex components decrease over time on U937 cells. (A+B) C1s, Factor B, and C1 inhibitor are lost from the surface of U937 cells after 48 h. U937 cells were grown in RPMI + 10% FBS (+  serum) or in Macrophage Serum-Free Media (-serum) for 48 h and stained with GaH C1q, C1s, C1r, or RaH C1-Inh, Factor B and isotype-matched controls followed by secondary Ab conjugated to Alexa 488. The cells were analyzed by flow cytometry. One experiment is shown (A), representative of three experiments. (B) *p ≤ 0.05; **p ≤ 0.01 (C) C1 complex components are released into the supernatant of U937 cells. Supernatants of U937 cell cultures were subjected to SDS-PAGE and subsequently visualized by Western blotting using the same primary Abs as above, followed by HRP-conjugated secondary Abs (n = 3).