Table 4.
Bacterial strains, plasmid and phages used in the study
| E.coli strains, plasmids and phages | Relevant Genotype | Reference |
|---|---|---|
| BL21-AI | F- ompT hsdSB(rB-, mB-) gal dcm (DE3), arabinose inducible T7 RNA polymerase | Invitrogen, Paisley, U.K. |
| MC1061 | F- Δ(ara-leu)7697 Δ(codB-lacI)3 galK16 λ- mcrA0 rpsL150(strR) mcrB1 | [18] |
| DM1187 | F- dam-13::Tn9(CmR) dcm- mcrB hsdR-M + gal1 ara- lac- thr- leu- tsxR | [45] |
| TOP10 | F- mcrA Φ80lacZΔM15 recA+ | Invitrogen, Paisley, U.K. |
| pCR®-Blunt | lacZ α, KanR, ccdB | Invitrogen, Paisley, U.K. |
| pET30c | Expression vector with T7 promoter, KanR, TetR, | Novagen, Notts, UK |
| Φ24B | Stx2-phage, ΔstxA2::aph3 | [14] |