Table 1.
Lifespan and intestinal colonization of C.elegans N2 and mutants with growth on E. colior Salmonellalawnsa
| E. coli OP50 | S. typhimuriumSL1344 | ||||
|---|---|---|---|---|---|
| Genotype | Symbol |
TD50 (Mean ± SD) |
Day 2 log10 intestinal cfu (Mean ± SD) |
TD50 (Mean ± SD) |
Day 2 log10 intestinal cfu (Mean ± SD) |
| N2 |  |
12.93 ± 0.50 | 2.76 ± 0.22 | 10.87 ± 1.37 | 3.22 ± 0.07 |
| daf-2 |  |
26.45 ± 1.34^^ | 1.70 ± 0.12^^ | 20.17 ± 0.29^^ | 1.87 ± 0.15^^ |
| age-1 |  |
18.75 ± 0.35^^ | 2.48 ± 0.32 | 13.70 ± 0.14^ | 2.36 ± 0.48^ |
| daf-16 |  |
8.05 ± 0.38^^ | 3.30 ± 0.19 | 5.53 ± 0.23^^ | 3.55 ± 0.15^ |
| lys-7 |  |
9.30 ± 0.74^ | 2.93 ± 0.39 | 8.83 ± 0.25^ | 3.31 ± 0.28 |
| spp-1 |  |
9.80 ± 0.59^ | 2.67 ± 0.27 | 8.70 ± 0.14^ | 3.41 ± 0.23 |
| sod-3 |  |
11.90 ± 1.01 | 2.87 ± 0.24 | 10.93 ± 1.23 | 3.45 ± 0.25 |
| ctl-2 |  |
9.48 ± 0.29^ | 2.69 ± 0.18 | 8.98 ± 0.67^ | 3.88 ± 0.14^ |
| dbl-1 |  |
5.80 ± 0.57^^ | 3.35 ± 0.06 | 4.75 ± 0.79^^ | 3.86 ± 0.19^ |
| lys-1 |  |
10.00 ± 0.40^ | 2.60 ± 0.22 | 8.95 ± 0.44^ | 3.12 ± 0.24 |
| pmk-1 |  |
7.40 ± 0.16^^ | 2.58 ± 0.34 | 6.10 ± 0.99^^ | 3.71 ± 0.78^ |
| tol-1 |  |
10.53 ± 0.31^^ | 2.81 ± 0.15 | 8.98 ± 0.79^ | 3.53 ± 0.18^ |
| trx-1 |  |
7.70 ± 0.14^^ | 2.95 ± 0.17 | 6.83 ± 0.38^^ | 3.30 ± 0.38 |
a Worms were age-synchronized by a bleaching procedure. Embryos were placed on mNGM agar plates containing E. coli OP50 or S. typhimurium SL1344 and incubated at 25°C. The L4 stage was designated as day 0. A total of 100 worms were used per lifespan assay. Bacterial colonization of the intestinal tract was determined at day 2 by washing and grinding 10 worms, and plating worm lysates on MacConkey agar. All assays were performed at least three times
^p< 0.05, compared to N2
^^p< 0.001, compared to N2