Table 5.
Primer used to verify microarray experiments in liver tissue by qRT-PCR
| Gene name | Probe-set ID | Sequence 5' - 3' | T(°C) | Size (bp) |
|---|---|---|---|---|
| CCND2 | Ssc.15749.1.S1_at | For AGGAGCAGATTGAGGTCGTG | 86 | 185 |
| Rev CAACCAGAGAGAAGGAAGGAGA | ||||
| NCAPG | Ssc.28512.1.S1_at | For CTTGTAGATTTGACGAGACCA | 60 | 156 |
| Rev GGCTTTAGTATAGACCCGAAC | ||||
| MGMT | Ssc.19639.1.A1_at | For GCAACTACTCGGGAGGAATG | 88 | 171 |
| Rev CTGCGAACGCTCAGTCTTG | ||||
| GADD45B | Ssc.14764.1.A1_at | For GGACTTAGACTTTGGGACTTG | 60 | 140 |
| Rev GTAAGCCTCCCATCTCTCTT | ||||
| SDHB | Ssc.8939.1.S1_at | For GCAGGACCCGTTCTCTCTGT | 60 | 170 |
| Rev GGTTACAGTCACGTTAGGTTGG | ||||
| PPARGC1A | Ssc.16864.1.S1_at | For GTAAATCTGCGGGATGATGG | 60 | 208 |
| Rev TGGTGGAAGCAGGATCAAAG | ||||
| PRKAA1 | Ssc.8107.1.A1_at | For TTGTTAATTTCATAAACTTTGCTTC | 60 | 193 |
| Rev GTGCAGCCTTGACATACTC | ||||
| PRKAA2 | Ssc.16257.1.S1_at | For TCTGTAATTCTGTTTTGCCTACGA | 60 | 168 |
| Rev AGCAAGAAGGTGATGCCAAG | ||||
| RPL10* | Ssc.9130.1.A1_at | For CTGTGTTCGTCTTTTCTTCC | 60 | 199 |
| Rev TCATCCACTTTTGCCTTCT |
CCND2 - cyclin D2; NCAPG - non-SMC condensin I complex, subunit G; MGMT - O-6-methylguanine-DNA methyltransferase; GADD45B - growth arrest and DNA-damage-inducible, β ; SDHB - succinate dehydrogenase complex, subunit B, iron sulfur (Ip); PPARGC1A - Peroxisome proliferator activated receptor γ coactivator-1α; PRKAA1 - 5'-AMP-activated protein kinase, catalytic α1 chain; PRKAA2 - 5'-AMP-activated protein kinase, catalytic α2 chain; RPL10 - Ribosomal protein 10; * house keeping gene.