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. 2011 Oct 12;2:50. doi: 10.3389/fimmu.2011.00050

Figure 3.

Figure 3

Localization and morphological phenotype of transgene-expressing stromal cells in LNs of Pdpn–Cre,R26-EYFP mice. (A) Histological section of inguinal LN from a Pdpn–Cre,R26-EYFP mouse stained for B cells (B220 in blue), Pdpn expressing stromal cells (red), and Cre-mediated transgene expression (EYFP, green). Inserts in the left panel mark the regions taken to acquire more detailed images in (B,C). Scale bar equals 200 μm. (B) Transgene-expressing stromal cells in the T cell zone with typical FRC structure marked with white arrow in the right panel. (C) Transgene-positive stromal cell in the subcapsular sinus (SCS) region visualized with anti-LyveI and anti-EYFP staining. Sinus-lining LEC is marked with white arrow in the right panel. Scale bars in (B,C) equal 10 μm.