Table 1.

Name, sequence and PCR conditions of the primers used

Name		Primer sequence	Annealing	Extension	Cycles
asl/outer	fw	GSKAARTTTAATGGKGCTGTWGG	47°C, 30 s	72°C, 50 s	35
	rv	GGATTAAYTTTATGAGGCATTG
asl/nested	fw	GCTGATMAAAATRTTGATTGG	50°C, 30 s	72°C, 30 s	38
	rv	GAGGCATTGTACTACTWCC
clpc/outer	fw	AAACTGAATTAGCAAAAATATTA	50°C, 30 s	72°C, 50 s	38
	rv	CGWGCWCCATATAAAGGAT
clpc/nested	fw	GATTTGATATGAGTGAATATATGG	48°C, 30 s	72°C, 30 s	40
	rv	CCATATAAAGGATTATAWG
coI/outer	fw	CTATTTATGGTTTTCATTTTTATTTGGTA	57°C, 30 s	72°C, 50 s	35
	rv	AGGAATACGTCTAGGCATTACATTAAATCC
coI/nested Po	fw	AGCAATATCAATAGCTGCATTACCT	62°C, 30 s	72°C, 50 s	38
	rv	GATTTTCTTCAATATAATGCCTGGA
cytb/outer	fw	TAATGCCTAGACGTATTCCTGATTATCCAG	55°C, 30 s	72°C, 50 s	35
	rv	TGTTTGCTTGGGAGCTGTAATCATAATGTG
cytb/nested	fw	TCAACAATGACTTTATTTGG	55°C, 30 s	72°C, 50 s	40
	rv	TGCTGTATCATACCCTAAAG

All denaturation and final extension periods are the same for all primer-pairs.