Table 1.
Identified SNPs within boTLR1 from Holstein Friesians.
| SNP | Genomic (bta6) position | Amino acid position | Amino acid | Restriction site presence | Previously reported | dbSNP ID |
|---|---|---|---|---|---|---|
| -1205A > G | 60440366 | - | - | - | No | - |
| -1151T > A | 60440312 | - | - | - | No | - |
| -79T > G | 60439240 | - | - | - | No | - |
| +798 C > T | 60438363 | 266 | Phe-Phe | MboII | Yes 1 | ss73689409 |
| +1641A > C | 60437520 | 547 | Ser-Ser | Bst X-I | Yes2 | ss73689413 |
| +1716 G > A | 60437445 | 572 | Lys-Lys | Bsi Y | Yes2 | ss73689414 |
| +1762A > G | 60437324 | 587 | Ile-Val | Bcl I | Yes2 | ss73689415 |
| +2100 C > T | 60436989 | 700 | Phe-Phe | - | Yes2 | ss73689417 |
| +2103 T > C | 60436986 | 701 | Val-Val | BsmAI | Yes2 | ss73689418 |
| +2463 C > T | 60436698 | - | - | MsII | No | - |
| +2731A > G | 60436430 | - | - | - | No | - |
Abbreviations: bta6 = Bos taurus chromosome 6, dbSNP = Single-Nucleotide Polymorphism Database [46]. All boTLR1 sequences were aligned with the published Btau_4.0 sequence NC_007304.4 (underlined allele denotes the published sequence). Light shading - synonymous coding SNPs, dark shading -non-synonymous coding SNP. All boTLR1 SNP offsets are given relative to their position (bp) from the A nucleotide of the first ATG codon. 1 [31], 2 [35].