Table 1. Initial semen traits and post-thaw sperm quality.
Boar 1 | Boar 2 | Boar 3 | Boar 4 | Boar 5 | |
Initial semen traits | |||||
Sperm concentration (×106/mL) | 254.1±21.1b (230–296) | 426.3±49.9a (330–497) | 247.1±11.5b (224–261) | 311.9±11.8ab (288–324) | 267.2±18.2b (232–294) |
Normal sperm morphology (%) | 98.3±1.7 (95–100) | 91.6±0.3 (91–92) | 97±0.6 (96–98) | 95±1.7 (92–98) | 99±0.6 (98–100) |
Total sperm motility (%) | 81.1±3.2 (76–87) | 78±1.2 (76–80) | 83.3±0.7 (82–84) | 73.7±3.2 (70–80) | 82.7±1.8 (79–85) |
Sperm viability (%) | 89.7±0.9x (88–91) | 80.3±2.3y (76–84) | 85±2.5xy (80–88) | 84.5±3.5xy .(77.5–89) | 83.3±1.2xy (81–85) |
Post-thaw sperm assessments | |||||
Total sperm motility (%) | 73.2±2.8a (67–81) | 47.3±2.6c (40–56) | 74.8±2.7a .(66–80) | 67.3±3.1ab (57–79) | 56.8±1.2bc (53–60) |
Sperm viability (%) | 73±1.8a (69–77.4) | 58±2.1c (51.5–66) | 75.2±1.8a (70.8–81) | 69.5±2.7ab (58.1–76) | 60.1±2.4bc (53.5–69) |
MDA generation (µmol/ 30×106 cells) | 7.6±1.1b (2.7–11.9) | 12.8±1.3a (7.6–18.7) | 11.6±0.7a (8.3–15.1) | 10.1±0.9ab (5–13.9) | 10.7±0.4ab (8.7–13.3) |
Superscripts indicate differences among boars: a–c p<0.01 and x–y p<0.05.
The initial semen traits and post-thaw sperm assessments of the native semen samples cryopreserved from the five boars. The data are the means ± SEMs of three ejaculates per boar, with ranges given between parentheses.