Table 1.
Match IDa | Protein description | Accession no.b | Protein score | Protein score CI%c | Mw (Da) | pI | Average ratio and p-value | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
P5-infected/control | P115-infected/control | |||||||||||||||||||||
4 dpi | 7 dpi | 14 dpi | 21 dpi | 4 dpi | 7 dpi | 14 dpi | 21 dpi | |||||||||||||||
ratio | p | ratio | p | ratio | p | ratio | p | ratio | p | ratio | p | ratio | p | ratio | p | |||||||
4467 | annexin A1 (ANXA1) | gi|46195459 | 910 | 100 | 38761 | 7.05 | 0.89 | 0.435 | 0.49 | 0.001 | ||||||||||||
2051 | B-creatine kinase | gi|211235 | 631 | 100 | 42525.5 | 5.78 | 1.44 | 0.025 | 1.77 | 0.006 | ||||||||||||
1926, 1736, 1817, 4581 |
cartilage matrix protein precursor | gi|71896317 | 314 | 100 | 54798.1 | 6.63 | 2.71 | 0.006 | 1.24 | 0.427 | 1.94 | 0.003 | 2.48 | 0.025 | 1.96 | 0.012 | 2.46 | 0 | ||||
1987 | fibrinogen beta chain | gi|211780 | 612 | 100 | 53271.8 | 7.18 | 2.75 | 0.006 | 2.57 | 0.011 | ||||||||||||
4679 | glutathione S-transferase class-alpha | gi|4959550 | 79 | 99.958 | 21326.5 | 8.03 | 1.36 | 0.005 | 1.8 | 0 | ||||||||||||
4036 | keratin, type I cytoskeletal 19 | gi|45384356 | 772 | 100 | 46111 | 4.94 | 2.43 | 0.016 | 2.15 | 0.009 | ||||||||||||
5150 | nmrA-like family domain-containing protein 1 | gi|71897147 | 653 | 100 | 32726 | 7.77 | 2.21 | 0.004 | 1.72 | 0.009 | ||||||||||||
4405 | PREDICTED: microfibril-associated glycoprotein 4 (EST) | gi|18470900 | 331 | 100 | 28600 | 5.2 | 0.57 | 0.001 | 0.97 | 0.695 | ||||||||||||
2021 | Vimentin (VIM) | gi|212868 | 1160 | 100 | 53166.6 | 5.09 | 0.76 | 0.015 | 0.56 | 0.001 |
a) Match ID represents unique numbers assigned to each spot in the matched standard of ImageMaster 2D Platinum version 6.0 software
b) Accession no. is the MASCOT result of MALDI-TOF/TOF searched from the NCBInr database
c) The criterion for a successfully identified protein is a protein score confidence interval (C.I. %) for PMF and MS/MS data ≥ 95%