Table 2.

Differential abundance changed proteins in tracheal from chicken infected with IBV ck/CH/LDL/97I P₅ and ck/CH/LDL/97I P₁₁₅

Match IDa	Protein description	Accession no.b	Protein score	Protein score CI%c	Mw (Da)	pI	Average ratio and p-value
							P5-infected/control								P115-infected/control
							4 dpi		7 dpi		14 dpi		21 dpi		4 dpi		7 dpi		14 dpi		21 dpi
							ratio	p	ratio	p	ratio	p	ratio	p	ratio	p	ratio	p	ratio	p	ratio	p
2079, 2083	aldehyde dehydrogenase 2 family	gi|118098552	983	100	64491.8	8.79	1.55	0.001							2.72	0.09
2751	aldo-keto reductase	gi|14330324	795	100	36790.8	7.63	0.99	0.05							2.48	0.079
2382	alpha-enolase	gi|46048768	611	100	47617.5	6.17	4.87	0.019							2.28	0.91
2481, 4391	annexin A2 (ANXA2)	gi|45382533	783	100	38901	6.92	1.54	0.371	N/A	0					2.44	0.012	4.63	0.003
2224	cartilage matrix protein precursor	gi|71896317	1090	100	54798.1	6.63	12.82	0.019							2.59	0
1689	creatine kinase M-type	gi|211528	443	100	43529.1	6.5			0.87	0.567							5.63	0.001
4195, 4953	fructose-bisphosphate aldolase C	gi|999392	256	100	39022.8	5.79			0.34	0.009			0.26	0			2.25	0.009			2.23	0.002
3273	heat shock protein beta-1 (HSPB1)	gi|45384222	766	100	21715	5.77	5.42	0.005							2.66	0.955
4317 4310, 4321	lamin-A	gi|45384214	651	100	73348.5	6.5							2.88	0							0.41	0.001
2592	nmrA-like family domain-containing protein 1	gi|71897147	1090	100	32726	7.77	0.56	0.001							2.78	0.003
2483	similar to D4-GDP-dissociation inhibitor (GDID4)	gi|50728568	552	100	22928.6	5.08					2.37	0.039							1.61	0.254
1932	similar to malate dehydrogenase 2, NAD (mitochondrial)	gi|50758110	819	100	37400.6	8.83			N/A	0.019							1.3	0.31
5132	similar to myozenin	gi|50749396	319	100	29589.7	7.85							2.24	0.058							N/A
1919, 1659	pyruvate kinase (PKM2)	gi|212571	291	100	58433.9	7.29	1.12	0.488	0.44	0.071	0.5	0.012			2.55	0.004	2.81	0.024	1.28	0.088
4635	selenium binding protein 1	gi|118102241	731	100	53097.6	6.17							0.82	0.156							1.86	0.034
2016	succinyl-CoA:3-ketoacid-coenzyme A transferase 1, mitochondrial	gi|60592998	201	100	56549.4	8.01	5.49	0.138							2.09	0
4680	UMP-CMP kinase	gi|71896025	947	100	22386.3	6.75			1.07	0.56							2.42	0.002
4786	vimentin (VIM)	gi|212868	1210	100	53166.6	5.09							4.78	0							1.4	0.394

a) Match ID represents unique numbers assigned to each spot in the matched standard of ImageMaster 2D Platinum version 6.0 software

b) Accession no. is the MASCOT result of MALDI-TOF/TOF searched from the NCBInr database

c) The criterion for a successfully identified protein is a protein score confidence interval (C.I. %) for PMF and MS/MS data ≥ 95%

N/A: A indicates the spot was detectable on one of the gels, N indicates the spot was too weak to detect on one of the gels