Table 4.
Master IDa | Protein description | Accession no.b | Protein score | Protein score CI%c | Mw (Da) | pI | Average ratio and p-value | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
P5-infected/control | P115-infected/control | |||||||||||||||||||||
4 dpi | 7 dpi | 14 dpi | 21 dpi | 4 dpi | 7 dpi | 14 dpi | 21 dpi | |||||||||||||||
ratio | p | ratio | p | ratio | p | ratio | p | ratio | p | ratio | p | ratio | p | ratio | p | |||||||
496 | aldehyde dehydrogenase 4 family, member A1 | gi|118101121 | 1010 | 100 | 112936 | 5.4 | 0.56 | 0.009 | 0.85 | 0.031 | ||||||||||||
662 | aldehyde dehydrogenase 4 family, member A1 | gi|118101121 | 890 | 100 | 112936 | 5.4 | 1.03 | 0.84 | 1.59 | 0.015 | ||||||||||||
452 | alpha-enolase | gi|46048768 | 353 | 100 | 47617.5 | 6.17 | 1.25 | 0.14 | 1.7 | 0.003 | ||||||||||||
821, 1088 |
alpha-tropomyosin of smooth muscle (TPM1) | gi|833618 | 94 | 99.999 | 32962.7 | 4.67 | 2.22 | 0.003 | 1.16 | 0.46 | ||||||||||||
1977 | apolipoprotein A-I (APOA1) | gi|211159 | 703 | 100 | 30673.2 | 5.58 | 0.97 | 0.31 | 1.58 | 0 | ||||||||||||
1262 | chain A, crystal structures of chicken annexin V in complex with Ca2+ (ANXA5) | gi|62738641 | 728 | 100 | 36158.6 | 5.61 | 2.04 | 0.004 | 1.1 | 0.75 | ||||||||||||
983, 784 |
chain C, crystal structure of native chicken fibrinogen | gi|8569623 | 921 | 100 | 47485.9 | 5.4 | 1.69 | 0.002 | 1.24 | 0.034 | 1.07 | 0.35 | 0.76 | 0.006 | ||||||||
1926 | low molecular weight phosphotyrosine proteinphosphatase (TCP1) | gi|86129490 | 304 | 100 | 18640.2 | 6.81 | 6.34 | 0.045 | 0.74 | 0.16 | ||||||||||||
1847 | manganese-containing superoxide dismutase precursor (MNSOD) | gi|12034955 | 258 | 100 | 25158.7 | 8.6 | 1.52 | 0.015 | 1.12 | 0.022 | ||||||||||||
1970 | nucleoside diphosphate kinase | gi|2827446 | 445 | 100 | 17542 | 7.11 | 4.68 | 0.003 | 1.48 | 0.81 | ||||||||||||
293 | phosphoenolpyruvate carboxykinase (EC 4.1.1.32) | gi|212538 | 492 | 100 | 70224.3 | 6.1 | 1.15 | 0.047 | 1.78 | 0 | ||||||||||||
665 | similar to aflatoxin aldehyde reductase | gi|118101125 | 286 | 100 | 36943.4 | 6.76 | 2.61 | 0.004 | 1.28 | 0.059 | ||||||||||||
789 | similar to betaine homocysteine methyltransferase | gi|50755288 | 1010 | 100 | 45552.1 | 7.56 | 2.51 | 1.41 | ||||||||||||||
582 | glutamate dehydrogenase 1, mitochondrial | gi|118534 | 339 | 100 | 56075.4 | 8.48 | 1.74 | 0.051 | 1.06 | 0.17 | ||||||||||||
1461, 2026 |
similar to methyltransferase 24 (MET24) | gi|50750103 | 556 | 100 | 30640.5 | 6 | 0.72 | 0.002 | 1.25 | 0.074 | 1.09 | 0.066 | 0.42 | 0 | ||||||||
987 | sulfotransferase | gi|45384226 | 265 | 100 | 36333.5 | 5.89 | 4.53 | 0.01 | 0.98 | 0.85 | ||||||||||||
1650 | triosephosphate isomerase (EC 5.3.1.1) | gi|212774 | 1290 | 100 | 26831.9 | 6.71 | 1.51 | 0.009 | 1.07 | 0.43 |
a) Match ID represents unique numbers assigned to each spot in the matched standard of Ettan™ DeCyder 2D v6.5 software
b) Accession no. is the MASCOT result of MALDI-TOF/TOF searched from the NCBInr database
c) The criterion for successful identification of a protein is a protein score confidence interval (C.I. %) for PMF and MS/MS data ≥ 95%