Figure 5. Protocadherin qRT-PCR and gene array analyses in activated Cm cells.

(5a) pcdh18 qRT-PCR analysis of FACS-purified Cm and Em cells from young, aged, or ‘memory’ mice (from Fig. 4d) was as indicated (bottom panels). Gene array of FACS-purified Cm cells from a pool of 10 aged mice was performed by the NYU Center for Health Informatics and Bioinformatics. Purified Cm were activated in vitro and total cells in each culture were isolated for RNA extraction. cDNAs were hybridized to GeneChip arrays using an Affymatrix platform and the data were processed as described in Materials and Methods. (5b) Bone marrow CD8⁺ T cells were purified by positive selection magnetic immunobeading from young or aged mice, activated in vitro with ConA, and qRT-PCR performed as before.