Table 1. Primers for qPCR.

GENE	5′→3′ SEQUENCE		qPCR Ta
PD-1L	FW	CCACGGAAATTGTCTGGTTG	60
PD-1L	RV	TGCTGCATAATCAGCTACGG	60
PCDH-19	FW	ACCATCACTTGTCTCCTCGGCTGT	58
PCDH-19	RV	CGTCCCGAGGTACAAGGCGG	58
DAB-2b	FW	CACTGATACCAGCCAATCCC	58
DAB-2b	RV	AATCAAGCTTGCAAGAGATTTGT	58
DAB1	RV	ACCAGCGCCAAGAAAGACT	58
DAB1	FW	ATCAGCTTGGCTTTGACCG	58
PCDH-12	FW	GTGAGGGGCAATGACAATCT	56
PCDH-12	RV	GAAGAGCTGTCGAGCCTGTT	56
PD-1	FW	CAGGCTGGGTAGAAGGTGAG	60
PD-1	RV	CATTCACTTGGGCTGTGCT	60
IFN	FW	AGCAACAGCAAGGCGAAAA	58
IFN	RV	CTGGACCTGTGGGTTGTTGA	58
GRANZYME-B	FW	CATGTAGGGTCGAGAGTGGG	58
GRANZYME-B	RV	CCTCCTGCTACTGCTGACCT	58
IL-2	FW	GTGCTCCTTGTCAACAGCG	58
IL-2	RV	GGGGAGTTTCAGGTTCCTGTA	58
DAB2a	RV	AAGTCATGCTGGCTCCACAG	58
DAB2a	FW	CACTGATACCAGCCAATCCC	58
GAPDH	FW	TGTGTCCGTCGTGGATCTGA	56
GAPDH	RV	CCTGCTTCACCACCTTCTTGA	56
TNF	FW	AGAGGCACTCCCCCAAAAGAT	58
TNF	RV	GCAGGAATGAGAAGAGGCTGA	58
PCDH-18	FW	TTTGGGGCGCGATTCTCCGA	56
PCDH-18	RV	GGACTCTGCACTCCTCCGTA	56
PCDH-8	FW	ATCTCGGGCCGAGAAGCTGA	56
PCDH-8	RV	CGGGGACACGTATCTGCTAC	56
b-ACTIN	FW	CCGTGAAAAGATGACCCAGATC	56
b-ACTIN	RV	CATACCCAGGAAGGAAGGCTG	56

(Ta = annealing temperature).