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. 2012 May 2;7(5):e35231. doi: 10.1371/journal.pone.0035231

Figure 3. A general RT-PCR approach with primers to exon 7a and 3'UTR failed to detect VEGFxxxb isoforms in human and mouse cDNA extracts.

Figure 3

RT-PCR reactions were performed using forward/reverse primers designed to amplify both VEGFxxx and VEGFxxxb isoforms from cDNAs extracted from mouse fibrosarcoma cell lines & tumours and normal mouse tissues (heart, lung, liver and kidney) as well as commercially sourced human tissue cDNAs. A, A single product corresponding to VEGF164/188 (194 bp) is evident in the panel of mouse cDNAs using forward and reverse primers designed to exon 7 and the 3'UTR (exon7/3′UTR) with no evidence of VEGF164b/188b (128 bp) products. B & C, A single product corresponding to VEGF165/189 (194 bp) is evident in human brain, bladder and kidney (AMS Biotechnology Ltd), and prostate and kidney (Primer Design Ltd) using forward and reverse primers designed to exon 7 (DB exon7a) and the 3'UTR (DB 3'UTR) respectively. No products corresponding to VEGF165b/189b (128 bp) are evident in any of these samples.